1. "Po' Lazarus" traditional James Carter and the Prisoners 4:31
2. "Big Rock Candy Mountain" McClintock Harry McClintock 2:16
3. "You Are My Sunshine" Davis, Mitchell Norman Blake 4:26
4. "Down to the River to Pray" traditional Alison Krauss 2:55
5. "I Am a Man of Constant Sorrow" (radio station version) Dick Burnett Soggy Bottom Boys & Dan Tyminski 3:10
6. "Hard Time Killing Floor Blues" James Chris Thomas King 2:42
7. "I Am a Man of Constant Sorrow" (instrumental) Burnett Norman Blake 4:28
8. "Keep On the Sunny Side" Blenkhorn, Entwisle The Whites 3:33
9. "I'll Fly Away" Brumley Alison Krauss & Gillian Welch 3:57
10. "Nobody but the Baby" traditional Emmylou Harris, Alison Krauss, Gillian Welch 1:57
11. "In the Highways" Carter Leah, Sarah, and Hannah Peasall 1:35
12. "I Am Weary, Let Me Rest" Roberts (Kuykendall) The Cox Family 3:13
13. "I Am a Man of Constant Sorrow" (instrumental) Burnett John Hartford 2:34
14. "O Death" traditional Ralph Stanley 3:19
15. "In the Jailhouse Now" Blind Blake, Rodgers Soggy Bottom Boys & Tim Blake Nelson 3:34
16. "I Am a Man of Constant Sorrow" (with band) Burnett Soggy Bottom Boys & Dan Tyminski 4:16
17. "Indian War Whoop" (instrumental) Hoyt Ming John Hartford 1:30
18. "Lonesome Valley" traditional The Fairfield Four 4:07
19. "Angel Band" traditional The Stanley Brothers 2:15